|
| Status |
Public on Apr 12, 2014 |
| Title |
B-cell lymphoma 140 |
| Sample type |
RNA |
| |
|
| Source name |
Tumoral sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: B-cell lymphoma
tumor type: Small Lymphocytic Lymphoma / Chronic Lymphocytic Leukemia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extraxted by trizol reagent (Invitrogen, Carlsbad, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were dephosphorilated, denaturalizated, treated with ligase T4 and labelled with Cy3 for 2 hours at 16ºC
|
| |
|
| Hybridization protocol |
Hybridization settings: 20 hours, at 55ºC, at 20rpm. Blocking agent, hybridization and washing buffers was performed according to agilent recomandation.
|
| Scan protocol |
Scan resolution 5um on an Agilent scanner
|
| Description |
US22502553_251643612274_S01_miRNA-v1_95_May07_2_4_CLL-G
|
| Data processing |
Data are normalized to the median of the series (between arrays) by R program version 2.4, (available at http://www.r-project.org/). For normalization was used the "Processed Signal" of the feature extraction files (already includes background subtraction) was used. Average of miRs replicates was then applied using the following web page: http://prep.bioinfo.cnio.es/. Significance Analysis of Microarray excel macro (SAM) was used.
|
| |
|
| Submission date |
Jul 20, 2010 |
| Last update date |
Apr 12, 2014 |
| Contact name |
Lorena Di Lisio |
| E-mail(s) |
[email protected]
|
| Organization name |
CNIO
|
| Street address |
C/ Melchor Fernández Almagro, 3
|
| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL9081 |
| Series (1) |
| GSE29493 |
microRNA signature in b-cell lymphomas and control human B cells from fresh tonsils |
|
