|
| Status |
Public on Dec 31, 2013 |
| Title |
Cortical neurons-Rot-24h-Rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Murine primary cortical neurons
|
| Organism |
Mus musculus |
| Characteristics |
strain: Swiss Albino mice
age: Gestation day 15-16
tissue: Primary cortical neurons
age of culture: Day 5
agent: 10nM rotenone
treatment time-point: 24h
|
| Treatment protocol |
Rotenone (95-98%, Sigma- Aldrich, R8875) was dissolved in DMSO to give a 10mM stock concentration which was stored at -20°C for not more than 6 months. The stock solution was further diluted down to 10uM in DMSO and added into NeuroBasal medium to obtain 10nM for drug treatment on neuron cultures. Neuronal samples then collected for global gene expression analyses.
|
| Growth protocol |
Neocortical neurons (gestational days 15 or 16) obtained from foetal cortices of Swiss albino mice were used to prepare the primary cultures employing previous described procedures with modifications [Cheung NS, Beart PM, Pascoe CJ, John CA, Bernard O. Human Bcl-2 protects against AMPA receptor-mediated apoptosis. J Neurochem. 2000; 74: 1613-1620.].
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and cleaned up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with E-gene HDA-GT12 genetic analyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol with a hybridization duration of 17h.
|
| Scan protocol |
Standard Illumina scanning protocol with a pmt value=478
|
| Description |
4165582011_F
Replicate 3
|
| Data processing |
The data were normalised using median normalisation with GeneSpring Ver 7.3
|
| |
|
| Submission date |
Jul 19, 2010 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Minghui Jessica Chen |
| Organization name |
Menzies Research Institute
|
| Department |
Neuroscience group
|
| Lab |
A/P Steve Cheung
|
| Street address |
Menzies Research Institute, University of Tasmania, Private Bag 24
|
| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7001 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE22997 |
Global gene profiling of rotenone-induced neuronal death |
|
