|
| Status |
Public on Dec 17, 2010 |
| Title |
DBY8268 60 min vs T0 - 5% EtOH |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
DBY8268 log growth
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: DBY8268
protocol: DBY8268 log growth
|
| Treatment protocol |
For ethanol-shocked cells, ethanol was added to a final concentration of 5% (v/v).
|
| Growth protocol |
All cells were grown for at least 8 doublings in log phase in YPD medium at 30C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
|
| Label |
Oyster 550 cyanine dye
|
| Label protocol |
Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
|
| |
|
| Channel 2 |
| Source name |
DBY8268 60 min after 5% EtOH shock
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: DBY8268
protocol: DBY8268 60 min after 5% EtOH shock
|
| Treatment protocol |
For ethanol-shocked cells, ethanol was added to a final concentration of 5% (v/v).
|
| Growth protocol |
All cells were grown for at least 8 doublings in log phase in YPD medium at 30C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
|
| Label |
Oyster cyanine 650 dye
|
| Label protocol |
Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
|
| |
|
| |
| Hybridization protocol |
As indicated in Gasch AP Methods in Enzymology 350: 393-414
|
| Scan protocol |
Arrays were scanned on an Axon 4000B scanner
|
| Description |
5% EtOH Time-course in DBY8268
|
| Data processing |
Data were extracted using GenePix 6.0. Data were normalized by regional mean-centering as described by Lyne et al. 2003 BMC Genomics
|
| |
|
| Submission date |
Jul 12, 2010 |
| Last update date |
Dec 17, 2010 |
| Contact name |
Jeffrey A. Lewis |
| E-mail(s) |
[email protected]
|
| Phone |
608-347-3661
|
| Organization name |
University of Arkansas
|
| Department |
Biological Sciences
|
| Lab |
Lewis Lab
|
| Street address |
850 W. Dickson St.
|
| City |
Fayetteville |
| State/province |
AR |
| ZIP/Postal code |
72701 |
| Country |
USA |
| |
|
| Platform ID |
GPL5915 |
| Series (1) |
| GSE22904 |
Comparison of the transcriptional response to ethanol in laboratory and wild yeast strains |
|
