|
| Status |
Public on Oct 13, 2021 |
| Title |
Hbo1 heterozygous control HBO1 (KAT7) |
| Sample type |
SRA |
| |
|
| Source name |
Bone marrow Progenitor cells
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
tissue: Bone marrow
cell type: LSK cells
gender: Male
genotype: Hbo1def/+; T/+
chip antibody: HBO1 (abcam 190908)
|
| Treatment protocol |
mice treated with poly(I:C) to induce Mx1-cre recombinase and generate Hbo1 KO and heterozygous controls
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells isolated by FACS
Libraires generated using Tn5 transposase during the CUT&Tag protocol
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Library strategy: CUT&Tag
FASTQ files were output using standard Illumina protocols and software.
Reads were aligned to the mouse genome by Rsubread version 2.0.1
Read counts were summarized by genomic regions - Gene bodies, TSS - 5 kb to TSS regions, and TES to TES + 5 kb regions - using the Rsubread's featureCounts function and mm10 NCBI annotation.
Genome_build: mm10 (NCBI build 38.1)
Supplementary_files_format_and_content: Tab-delimited text files for gene body counts; columns represent Entrez Gene ID, total gene length (total base count of all exons) and the number of reads mapping to that gene. Tab-delimited text files for other region counts around TSS and TES of genes; columns represent Entrez Gene ID, and the number of reads mapping to that region.
|
| |
|
| Submission date |
Oct 13, 2021 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Waruni Abeysekera |
| E-mail(s) |
[email protected]
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE185820 |
The histone lysine acetyltransferase HBO1 regulates hematopoietic stem cell quiescence and self-renewal |
|
| Relations |
| BioSample |
SAMN22245314 |
| SRA |
SRX12591520 |
