|
| Status |
Public on Oct 01, 2021 |
| Title |
atg5_T30_C2_LN |
| Sample type |
RNA |
| |
|
| Source name |
atg5 30 days growth under LN culture 2
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: atg5 mutant
organ: whole rosette
nutrition: low nitrate
|
| Growth protocol |
Plants were grown under low nitrate (2 mM) or high nitrate (10 mM) for 30 and 60 days after sowing in short days (160 micromol.m-2.s-1; 8 h light, 16 h dark) (www.plantcell.org/cgi/doi/10.1105/tpc.114.124677)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Microarray hybridations were performed at RIKEN Plant Science Center (Yokohama Japan) using ATH1 microarrays according to manufacturer's instructions.
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Gene expression data from atg5 rosette, in low nitrate at 30 days after sowing
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 30, 2021 |
| Last update date |
Oct 01, 2021 |
| Contact name |
Celine Masclaux-Daubresse |
| E-mail(s) |
[email protected]
|
| Phone |
+33130833088
|
| Organization name |
IJPB INRAE
|
| Street address |
Route de Saint Cyr
|
| City |
Versailles |
| ZIP/Postal code |
78000 |
| Country |
France |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE185085 |
Expression data from Arabidopsis autophagy mutants (atg5 and atg9) and wild type (Col-0) |
|
