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Sample GSM560541 Query DataSets for GSM560541
Status Public on Sep 09, 2010
Title 04-H
Sample type RNA
 
Source name Monocyte-derived dendritic cells
Organism Homo sapiens
Characteristics cell type: Monocyte-derived dendritic cells
virus: HIVGFP(G)
Treatment protocol 100,000 MDDC were plated in 96 well U bottom plate in the presence of 5 micrograms/ml of polybrene. MDDC were infected with HIVGFP(G) and SIVVLP(G) supernatants that were generated by transient transfection of 293T cells. Celles were harvested 48 hours after infection.
Growth protocol Human CD14+ monocytes were isolated from buffy coats of adult peripheral blood using a Ficoll gradient and two sequential purifications with anti-CD14 magnetic beads (Miltenyi). Purified monocytes were cultured in the presence of GM-CSF and IL-4 for 4 days in RPMI media containing 10% fetal bovine serum and antibiotics.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol According to Affymetrix protocols
 
Hybridization protocol According to Affymetrix protocols
Scan protocol According to Affymetrix protocols
Description Donor 2
Data processing Data was analyzed using R version 2.6.0 (r43408) and Bioconductor packages "simpleaffy" (version 2.14.05) and "hgu133plus2" (version 2.0.1) with the MAS 5.0 algorithm.
 
Submission date Jun 27, 2010
Last update date Aug 31, 2010
Contact name Dan Littman
E-mail(s) [email protected]
Organization name NYU Medical Center
Street address 540 1st ave
City New York
State/province NY
ZIP/Postal code 10016
Country USA
 
Platform ID GPL570
Series (1)
GSE22589 A cryptic sensor for HIV-1 activates antiviral innate immunity in dendritic cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 6.257144479
1053_at 7.418131224
117_at 7.154383617
121_at 7.638915833
1255_g_at 5.885211754
1294_at 7.002447479
1316_at 4.13526953
1320_at 0.325121034
1405_i_at 6.127541821
1431_at 2.261104808
1438_at 1.738959966
1487_at 7.64787103
1494_f_at 4.706681841
1552256_a_at 9.446109359
1552257_a_at 7.365997514
1552258_at 4.335766105
1552261_at 3.654967498
1552263_at 9.324504181
1552264_a_at 9.019809289
1552266_at 2.876293827

Total number of rows: 54675

Table truncated, full table size 1216 Kbytes.




Supplementary file Size Download File type/resource
GSM560541.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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