|
| Status |
Public on Sep 09, 2010 |
| Title |
03-H |
| Sample type |
RNA |
| |
|
| Source name |
Monocyte-derived dendritic cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Monocyte-derived dendritic cells
virus: HIVGFP(G)
|
| Treatment protocol |
100,000 MDDC were plated in 96 well U bottom plate in the presence of 5 micrograms/ml of polybrene. MDDC were infected with HIVGFP(G) and SIVVLP(G) supernatants that were generated by transient transfection of 293T cells. Celles were harvested 48 hours after infection.
|
| Growth protocol |
Human CD14+ monocytes were isolated from buffy coats of adult peripheral blood using a Ficoll gradient and two sequential purifications with anti-CD14 magnetic beads (Miltenyi). Purified monocytes were cultured in the presence of GM-CSF and IL-4 for 4 days in RPMI media containing 10% fetal bovine serum and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix protocols
|
| |
|
| Hybridization protocol |
According to Affymetrix protocols
|
| Scan protocol |
According to Affymetrix protocols
|
| Description |
Donor 1
|
| Data processing |
Data was analyzed using R version 2.6.0 (r43408) and Bioconductor packages "simpleaffy" (version 2.14.05) and "hgu133plus2" (version 2.0.1) with the MAS 5.0 algorithm.
|
| |
|
| Submission date |
Jun 27, 2010 |
| Last update date |
Aug 31, 2010 |
| Contact name |
Dan Littman |
| E-mail(s) |
[email protected]
|
| Organization name |
NYU Medical Center
|
| Street address |
540 1st ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE22589 |
A cryptic sensor for HIV-1 activates antiviral innate immunity in dendritic cells |
|
