|
| Status |
Public on Jun 24, 2010 |
| Title |
1216-4HF |
| Sample type |
RNA |
| |
|
| Source name |
High fat maternal diet, Cited2 -/-
|
| Organism |
Mus musculus |
| Characteristics |
maternal diet: High fat
genotype/variation: Cited2 -/-
strain: C57BL6/J
developmental stage: Embryonic (8.5 days post coitum)
|
| Treatment protocol |
Embryos were harvested at 8.5 dpc in chilled DEPC-PBS, dissected out of the yolk sac, the allantois was removed, somites counted for the purposes of stage-matching, then flash frozen in liquid nitrogen.
|
| Growth protocol |
Female Cited2+/— mice were fed with Beekay Rat and Mouse Diet (BK002P) between weaning and 6 weeks of age, and then were randomly assigned to specially formulated high-fat or control diets, which were provided ad libitum. At baseline and after 8 weeks on the diet, the mice were weighed and a 6-hour fasting blood sample was collected. Blood glucose and plasma insulin were measured. Following 8 weeks on diet, Cited2+/— female mice were mated with Cited2+/— males, and the diet continued through pregnancy.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Micro Kit was used as per manufacturer's instructions. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina labelling protocol was used to prepare biotinylated cRNA
|
| |
|
| Hybridization protocol |
Samples were hybridised for 16 hours to an Illumina Sentrix Mouse Whole Genome Ref 6 v2 chip. Washing, staining and scanning was performed according to the manufacturer’s protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Embryonic_High fat maternal diet, Cited2 -/-_replicate3
|
| Data processing |
Data generated from scanning was imported into Illumina BeadStudio version 3.2.6 to generate signal values for each bead type. These data were exported to the R statistical computing language where it was background corrected, and transformed and normalised using the BioConductor package, vsn2 (Huber et al. 2002).
|
| |
|
| Submission date |
Jun 24, 2010 |
| Last update date |
Jun 24, 2010 |
| Contact name |
Shoumo Bhattacharya |
| E-mail(s) |
[email protected]
|
| Organization name |
Wellcome Centre For Human Genetics, University of Oxford
|
| Department |
RDM Cardiovascular Medicine
|
| Street address |
Roosevelt Drive
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7BN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE22558 |
Effects of a high-fat maternal diet and embryonic Cited2 genotype on gene expression and development in mouse |
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