|
| Status |
Public on Jun 30, 2010 |
| Title |
Dmrt1 KO NGN-cre 12504702_532 |
| Sample type |
RNA |
| |
|
| Source name |
P9 testes
|
| Organism |
Mus musculus |
| Characteristics |
tissue: juvenile testes
cre: Cre-positive
genotype: NGN-cre
age: P9
sex: Male
genotype: Dmrt1 KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from P9 testes using TRIzolTM reagent (Life Technologies), further purified with the RNeasy minelute cleanup kit (Qiagen 74204), and RNA quality was verified with a 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Standard Nimble protocol: RNA was reverse transcribed and hybridized to an MM8_60mer_expr array (NimbleGen A4543-00-01)
|
| |
|
| Hybridization protocol |
standard Nimblegen protocol
|
| Scan protocol |
standard Nimblegen protocol
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jun 22, 2010 |
| Last update date |
Jun 22, 2010 |
| Contact name |
Aaron Sarver |
| Organization name |
University of Minnesota
|
| Department |
Masonic Cancer Center
|
| Lab |
Biostatistics and Informatics
|
| Street address |
425 East River Road
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL10572 |
| Series (2) |
| GSE22507 |
Conditional Dmrt1 knockout gene expression (phase 7) |
| GSE22510 |
Conditional Dmrt1 knockout gene expression |
|
