|
| Status |
Public on Aug 30, 2010 |
| Title |
IPAH_89 |
| Sample type |
RNA |
| |
|
| Source name |
PMBCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PMBCs
disease state: Idiopathic Pulmonary Arterial Hypertension
gender: Female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Collection protocol: Whole blood was drawn from each subject via peripheral venipuncture into BD Vacutainer® CPT™ tubes with sodium citrate.
Isolation protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by centrifugation, and cells were stored in RNALater (Qiagen Inc., Valencia, CA) at -80°C.
Total RNA was isolated from PBMCs using Trizol (Invitrogen, Corporation, Carlsbad, CA) extraction and isopropyl alcohol precipitation, followed by clean-up with a Qiagen RNeasy mini-kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Microarray targets were prepared from 5µg of total RNA using the Affymetrix (Santa Clara, CA) kit for reverse transcription and a single round of in vitro transcription. HG-U133+2.0 were hybridized with 10µg of cRNA and processed per the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
gene expression data
IPAH_89_3
|
| Data processing |
Raw data was normalized using RMA and presented as non-log transformed signal intensity
|
| |
|
| Submission date |
Jun 15, 2010 |
| Last update date |
Jun 15, 2010 |
| Contact name |
Christopher D Coldren |
| E-mail(s) |
[email protected]
|
| Phone |
303 724 6056
|
| Organization name |
University of Colorado School of Medicine
|
| Department |
Medicine
|
| Lab |
Pulmonary Sciences and Critical Care Medicine
|
| Street address |
12700 East 17th Place
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE22356 |
Altered immune phenotype in peripheral blood cells of patients with scleroderma-associated pulmonary hypertension |
| GSE76809 |
Multi-tissue functional genomic study of systemic sclerosis |
|
