|
| Status |
Public on Jun 19, 2010 |
| Title |
S.R. K562, BR2, 36hr |
| Sample type |
RNA |
| |
|
| Source name |
cell line K562
|
| Organism |
Homo sapiens |
| Characteristics |
time point: 36 hr
agent: calcitriol
cell line: K562
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Exiqon RNA extraction kit according to manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the WT Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HuGene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G
|
| Description |
36 hour calcitriol stimulated
|
| Data processing |
The data were analyzed with Affymetrix Expression Console ver. 1.0. using Affymetrix default analysis settings and RMA normalisation.
|
| |
|
| Submission date |
Jun 07, 2010 |
| Last update date |
Jun 18, 2010 |
| Contact name |
Thomas Thykjaer |
| E-mail(s) |
[email protected]
|
| Organization name |
AROS Applied Biotechnology
|
| Street address |
IncubaSciencePark Skejby
|
| City |
Aarhus N |
| ZIP/Postal code |
8220 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE22174 |
Vitamin D and Gene Expression [A690] |
| GSE22176 |
Vitamin D and Gene Expression |
|
