|
| Status |
Public on Aug 17, 2021 |
| Title |
Blood_healthy_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood,healthy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
gender: male
age: 56
|
| Treatment protocol |
Ethylenediaminetetraacetic acid (EDTA)-treated whole blood samples from depression patients and healthy volunteers were collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified usingPAXgeneTM Blood RNA Kit (Cat#762174, QIAGEN, GmBH, Germany) following the manufacturer’s instructions.Then quantified by the NanoDrop ND-2000 (Thermo Scientific)and the RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
total RNA were dephosphorylated, denaturated and then labeled with Cyanine-3-CTP.
|
| |
|
| Hybridization protocol |
After purification the labeled RNAs were hybridized onto the microarray.
|
| Scan protocol |
After washing, the arrays were scanned with the Agilent Scanner G2505C (Agilent Technologies)
|
| Description |
gene ecpression in healthy people
|
| Data processing |
Feature Extraction software (version10.7.1.1, Agilent Technologies) was used to analyze array images to get raw data. Next, Genespring software (version 14.8, Agilent Technologies) was employed to finish the basic analysis with the raw data. To begin with, the raw data was normalized with the quantile algorithm.
|
| |
|
| Submission date |
Aug 16, 2021 |
| Last update date |
Aug 17, 2021 |
| Contact name |
Ting Zhou |
| E-mail(s) |
[email protected]
|
| Organization name |
Fengxian Hospital, Southern Medical University
|
| Street address |
No.6600, Nanfeng highway, Fengxian District
|
| City |
Shanghai |
| ZIP/Postal code |
201499 |
| Country |
China |
| |
|
| Platform ID |
GPL24741 |
| Series (2) |
| GSE182194 |
Development depression-associated miRNA biomarkers research |
| GSE182195 |
Development depression-associated biomarkers research |
|
