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Sample GSM5490 Query DataSets for GSM5490
Status Public on Apr 05, 2003
Title Spleen vs CD27 Treated B cell 60min (set3)
Sample type RNA
 
Channel 1
Source name Spleen was labeled with Cyanine-3 (green).
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name CD27 Treated B cells were labeled with Cyanine-5 (red)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD27 for 60min
 
Submission date Apr 04, 2003
Last update date May 27, 2005
Contact name Sangdun Choi
E-mail(s) [email protected]
Phone 626-395-8732
URL http://www.signaling-gateway.org
Organization name California Institute of Technology
Department Biology
Lab Alliance for Cellular Signaling Molecular Biology Laboratory
Street address 1200 E. California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platform ID GPL260
Series (2)
GSE375 Spleen vs CD27 Treated B cell
GSE2050 Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands

Data table header descriptions
ID_REF Feature number which correpond to the order of genes on the array
RATIO Raw Log10 Ratio
G_PROCESSED Green Processed signal, after dye normalization and background subtraction, used for computation of log ratio
R_PROCESSED Red Processed signal, after dye normalization and background subtraction, used for computation of log ratio
G_MEAN Green Channel Mean Intensity
R_MEAN Red Channel Mean Intensity
G_MEDIAN Green Channel Median Intensity
R_MEDIAN Red Channel Median Intensity
G_SD Green Channel Standard Deviation
R_SD Red Channel Standard Deviation
G_BG_MEAN Green Channel Mean Background Level
R_BG_MEAN Red Channel Mean Background Level
G_BG_MEDIAN Green Channel Median Background Level
R_BG_MEDIAN Red Channel Median Background Level
G_BG_SD Green Channel Background Standard Deviation
R_BG_SD Red Channel Background Standard Deviation
VALUE Processed Log2 Ratio after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots.

Data table
ID_REF RATIO G_PROCESSED R_PROCESSED G_MEAN R_MEAN G_MEDIAN R_MEDIAN G_SD R_SD G_BG_MEAN R_BG_MEAN G_BG_MEDIAN R_BG_MEDIAN G_BG_SD R_BG_SD VALUE
1 -0.102114 53674.9 42428.5 65516.6 65516.5 65516 65516 0.791695 0.642733 875.993 1219.94 873 1218 33.983 55.7046 0
2 -0.126124 187.411 140.175 1016.04 1376.04 1016 1372 36.7406 86.572 860.954 1205 860 1213 25.7597 58.7373 0
3 0.184504 75.7933 115.914 905.259 1334.04 905 1336 52.095 84.4651 869.162 1196.24 867 1195 33.1363 61.9549 0
4 -0.126363 76.2337 56.9881 908.92 1236.12 911 1219 33.0907 80.4764 873.631 1205.59 871 1202 30.0073 59.9113 0
5 0.121913 58.5945 77.5836 903.192 1285.19 900 1296 22.0327 61.6792 884.988 1216.06 884.5 1216 31.1011 62.3105 0
6 -0.0381657 547.895 501.801 1503 1925.5 1509.5 1922 78.7915 132.088 900.931 1217.84 901 1213 28.4546 58.7455 0
7 0.078474 65.052 77.9354 921.593 1267.07 919 1255 26.3121 80.2012 896.957 1197.62 896.5 1199.5 30.0789 54.619 0
8 -0.0295628 38.7127 36.1652 913.64 1215 907 1211 32.7183 46.4112 915.644 1222.11 915 1215 30.9348 57.4652 0
9 0.281892 6087.78 11650.7 8826.26 17529.7 8750 17483 637.845 1603.26 943.095 1237.48 933 1223 40.7662 89.0537 0.936424955
10 0.359679 723.336 1655.84 1768.81 3624.62 1681.5 3244.5 322.042 968.23 931.216 1202.64 927 1199 32.4459 66.9522 1.194827775
11 -0.05779 33.2558 29.1124 925.4 1180.04 921 1183 22.3774 59.1231 932.8 1200.48 931 1199 26.4618 56.3939 0
12 -0.000700144 51.5124 51.4295 946.32 1236.52 946 1244 15.0187 47.8445 935.561 1215.46 934 1208 25.882 60.4332 0
13 0.0154445 51.4192 53.2807 948.926 1251.11 944 1242 29.3597 58.9559 938.223 1226.6 933 1232 28.0038 58.8665 0
14 0.268006 914.737 1695.52 2022 3709.88 2052.5 3806.5 169.512 406.363 944.088 1247.33 941.5 1244.5 27.6578 47.7428 0.890296661
15 0.107161 2504.61 3205.54 4103.88 5766.31 2673.5 3733.5 2819.7 4054.06 952.313 1242.1 950 1240 28.8472 65.5961 0
16 0.344579 1604.67 3547.86 2954.42 6320.15 2537.5 5163.5 982.242 2499.35 962.939 1270.1 960 1267 32.7259 64.9319 0
17 -0.372167 834.172 354.07 1900.61 1735.91 1585 1637 696.905 275.563 962.195 1260.02 960 1254.5 31.3438 65.0993 0
18 0.0513689 453.989 510.993 1457.75 1968.79 1459 1974 71.5524 116.199 967.559 1243.68 967 1248 27.7464 56.319 0.170643792
19 0.445451 5421.22 15119.9 7974.85 22405.6 8310 23507 1255.9 3716.15 961.987 1230.38 962.5 1236.5 23.7847 56.5901 1.479756192
20 -0.0719211 6336.02 5369.02 9135.89 8721.5 9272.5 8782.5 1366.46 1193.65 963.969 1223.35 965 1225.5 28.9752 61.4116 -0.238916723

Total number of rows: 16273

Table truncated, full table size 1997 Kbytes.




Supplementary data files not provided

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