|
| Status |
Public on Mar 08, 2011 |
| Title |
Pineal_CT6_D_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Chicken pineal gland, CT6, dark
|
| Organism |
Gallus gallus |
| Characteristics |
gender: male
tissue: pineal gland
age: 8 days
time: CT6
treatment: dark
|
| Treatment protocol |
The animals were exposed to light for 1 h from CT (circadian time) 6 (representing subjective day), CT14 (early subjective night) or CT22 (late subjective night). Control animals were kept in the dark without light pulse.
|
| Growth protocol |
12-h light/12-h dark cycle-entrained chicks were transferred to constant darkness for a day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from the isolated pineal glands by using TRIZOL reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Nine micrograms of total RNA from 6 pineal glands was used to synthesize biotinylated cRNA by using Affymetrix GeneChip one-cycle cDNA synthesis kit and IVT labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Twenty micrograms of biotinylated cRNA was hybridized to GeneChip Chicken Genome Array for 16 h at 45°C. The arrays were washed and stained by using the Affymetrix Model 450 Fluidics Station.
|
| Scan protocol |
Arrays were scanned by the Affymetrix Model 3000 scanner.
|
| Description |
CT6_D_2
|
| Data processing |
The scanned array image was processed by GeneChip Operating Software Version 1.4 (Affymetrix) to calculate the signal intensity data for each probe set.
|
| |
|
| Submission date |
May 19, 2010 |
| Last update date |
Mar 09, 2011 |
| Contact name |
Yoshitaka Fukada |
| Organization name |
The University of Tokyo
|
| Street address |
Hongo 7-3-1
|
| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE21915 |
Time-of-day-dependent light-induction of gene expression in the chicken pineal gland |
|
