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Sample GSM5447 Query DataSets for GSM5447
Status Public on Apr 05, 2003
Title Spleen vs Anti-IgM Treated B cell 120min (set2)
Sample type RNA
 
Channel 1
Source name Spleen was labeled with Cyanine-3 (green).
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Anti-IgM Treated B cells were labeled with Cyanine-5 (red)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Anti-IgM for 120min
 
Submission date Apr 04, 2003
Last update date May 27, 2005
Contact name Sangdun Choi
E-mail(s) [email protected]
Phone 626-395-8732
URL http://www.signaling-gateway.org
Organization name California Institute of Technology
Department Biology
Lab Alliance for Cellular Signaling Molecular Biology Laboratory
Street address 1200 E. California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platform ID GPL260
Series (2)
GSE371 Spleen vs Anti-IgM Treated B cell
GSE2050 Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands

Data table header descriptions
ID_REF Feature number which correpond to the order of genes on the array
RATIO Raw Log10 Ratio
G_PROCESSED Green Processed signal, after dye normalization and background subtraction, used for computation of log ratio
R_PROCESSED Red Processed signal, after dye normalization and background subtraction, used for computation of log ratio
G_MEAN Green Channel Mean Intensity
R_MEAN Red Channel Mean Intensity
G_MEDIAN Green Channel Median Intensity
R_MEDIAN Red Channel Median Intensity
G_SD Green Channel Standard Deviation
R_SD Red Channel Standard Deviation
G_BG_MEAN Green Channel Mean Background Level
R_BG_MEAN Red Channel Mean Background Level
G_BG_MEDIAN Green Channel Median Background Level
R_BG_MEDIAN Red Channel Median Background Level
G_BG_SD Green Channel Background Standard Deviation
R_BG_SD Red Channel Background Standard Deviation
VALUE Processed Log2 Ratio after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots.

Data table
ID_REF RATIO G_PROCESSED R_PROCESSED G_MEAN R_MEAN G_MEDIAN R_MEDIAN G_SD R_SD G_BG_MEAN R_BG_MEAN G_BG_MEDIAN R_BG_MEDIAN G_BG_SD R_BG_SD VALUE
1 -0.158828 65002.4 45092.2 65516.8 65516.7 65517 65516.5 0.633671 0.788377 805.416 1161.71 808 1160 38.248 62.4008 0
2 -0.390031 111.563 45.4453 843.192 1071.12 839.5 1049 36.876 70.1617 786.988 1125.57 785 1124 35.9394 68.0706 0
3 -0.0971734 76.1438 60.8781 808.407 1132.67 798 1138 49.7279 69.6552 781.255 1121.21 779 1121 30.8539 72.6692 0
4 -0.316522 77.5801 37.4307 776.08 963.52 777 956 32.8886 58.8643 772.846 1117.9 770 1115.5 33.3407 77.913 0
5 -0.0257086 56.0973 52.8729 784.357 1132.54 778 1147 32.4097 75.6015 778.83 1130.52 776 1128 33.1057 75.1886 0
6 -0.460653 391.67 135.602 1159.04 1192.19 1148 1180 73.3676 77.5837 773.731 1092.7 770 1096.5 32.0824 65.5183 0
7 -0.142557 60.0751 43.2651 766.28 1050.84 758 1054 50.1593 71.2745 757.344 1063.92 756 1061 27.5178 62.9693 0
8 -0.0213979 57.9767 55.1894 750.667 1039.96 746.5 1034.5 18.2439 49.5269 743.049 1034.93 740 1033 25.2834 59.8426 0
9 0.260902 3809.38 6946.35 5062.63 9604.29 5132.5 9586.5 209.144 409.153 759.805 1024.41 750.5 1024.5 41.6349 66.6791 0.866697684
10 0.549021 851.493 3014.41 1670.54 4702.39 1562.5 4542.5 383.757 1976.62 750.099 1010.82 746 1013.5 35.3337 78.2364 0
11 0.132738 63.2888 85.9141 758.32 1052.92 751 1052 33.9113 46.1275 743.606 1007.25 740 1009 32.8025 73.6266 0
12 -0.0798432 74.1644 61.7096 777.773 1044.86 778.5 1050 33.3137 48.4751 752.673 1032.18 752 1026 34.9094 66.1478 0
13 -0.0347378 57.0647 52.678 755.12 1019.32 747 1022 34.0824 36.4757 748.597 1017.64 745.5 1009.5 33.9435 62.2144 0
14 0.353549 706.986 1595.73 1480.11 2964.04 1459 2961 120.384 326.536 746.034 989.601 740 988.5 40.3086 65.0242 1.174464356
15 0.205702 1176.83 1889.79 2023.6 3261.48 1010 1478 2023.06 3695.34 738.463 964.231 736 962 29.3937 52.1992 0
16 0.245299 1312 2307.99 2181.52 3760.43 1860 3168 768.698 1642.84 729.607 964.313 726.5 959 28.3516 56.405 0
17 -0.508247 721.639 223.91 1471.09 1151.39 1352 1142 416.436 91.7401 729.623 934.974 726 933 31.2589 53.6498 0
18 0.288461 265.795 516.425 969.407 1509.96 965 1524 51.8565 128.323 726.854 915.921 723 922 26.6099 54.0682 0.9582467
19 0.468348 4034.19 11860.5 5222.75 15868.3 5272 15615.5 1079.29 4097.8 730.755 945.806 731 944 24.9809 57.4227 1.555818379
20 -0.171188 6254.13 4216.76 7825.86 6104 8036 6240 1025.55 1032.77 729.093 925.192 727 920 28.33 53.5945 -0.568674227

Total number of rows: 16273

Table truncated, full table size 1966 Kbytes.




Supplementary data files not provided

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