|
| Status |
Public on May 14, 2010 |
| Title |
PBMCs rep 2 activated |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs healthy donor activated
|
| Organism |
Homo sapiens |
| Characteristics |
protocol: PBMC activation
|
| Treatment protocol |
Buffy coats were separated in Ficoll gradient, supernatant was discarded and layer containing PBMCs carefully collected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol RNA isolation following manufectureres guidlines
|
| Label |
biotin
|
| Label protocol |
Standard Affymentrix protocol (IFG Münster, Germany)
|
| |
|
| Hybridization protocol |
Standard Affymentrix protocol (IFG Münster, Germany)
|
| Scan protocol |
Standard Affymentrix protocol (IFG Münster, Germany)
|
| Description |
Probe5_ly19_akt6_070306
|
| Data processing |
RMA processing/normalization.
Using Array Assist 4.0 (Stratagene), filtering genes with change in expression with significance of p<0.05 and a fold change >2 (including Bonferroni correction).
|
| |
|
| Submission date |
May 14, 2010 |
| Last update date |
May 14, 2010 |
| Contact name |
Christoph Ogrodowczyk |
| Organization name |
ZMNH
|
| Department |
Institute for Neural Signal Transduction
|
| Street address |
Falkenried 94
|
| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE21837 |
Expression data from unactivated vs. activated PBMCs |
|
