|
| Status |
Public on May 16, 2011 |
| Title |
Male_Midgut_1 |
| Sample type |
RNA |
| |
|
| Source name |
Anopheles male midgut
|
| Organism |
Anopheles gambiae |
| Characteristics |
stage: adult
tissue: midgut
gender: male
|
| Treatment protocol |
Mosquitoes were anaesthetised briefly by chilling on ice, then immediately dissected.
|
| Growth protocol |
5 day old male and female mosquitoes. Samples for males and females where blooded after the bloodmeal over at three time points over 72hrs (24hrs, 48hr, 72hrs)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard two-cycle Affymetrix protocol from 50ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on GeneChip Plasmodium/Anopheles Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip scanner 3000 7G.
|
| Description |
Anopheles male midgut
|
| Data processing |
The data were analyzed with [R] Bioconductor using the GC-RMA normalization and MAS probe detection libraries.
|
| |
|
| Submission date |
May 05, 2010 |
| Last update date |
Jan 03, 2012 |
| Contact name |
Dean Baker |
| Organization name |
University of Cambridge
|
| Street address |
Department of Genetics, Downing Street
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 3EH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE21689 |
MozAtlas: A comprehensive gene expression atlas of sex- and tissue-specificity in the malaria vector, Anopheles gambiae. |
|
