|
| Status |
Public on May 12, 2010 |
| Title |
Sz patient R30 |
| Sample type |
other |
| |
|
| Channel 1 |
| Source name |
purified CD4+ from peripheral blood from Sz patient
|
| Organism |
Homo sapiens |
| Characteristics |
age: 77
gender: male
cd4 count: 3105
cd4+/cd8+: 25.65
|
| Extracted molecule |
other |
| Extraction protocol |
MicroRNA was isolated from samples using Trizol (Invitrogen, Paisley, UK) and RNeasy columns as described by the manufacturer (Qiagen, Crawley, UK).
|
| Label |
cy3
|
| Label protocol |
Enriched microRNA (500ng) samples were labeled with either Cy3 or Cy5 dyes using an Array 900microRNA direct kit from Genisphere (Hatfield, PA)
|
| |
|
| Channel 2 |
| Source name |
tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: pooled tonsils (n=12)
|
| Extracted molecule |
other |
| Extraction protocol |
MicroRNA was isolated from samples using Trizol (Invitrogen, Paisley, UK) and RNeasy columns as described by the manufacturer (Qiagen, Crawley, UK).
|
| Label |
cy5
|
| Label protocol |
Enriched microRNA (500ng) samples were labeled with either Cy3 or Cy5 dyes using an Array 900microRNA direct kit from Genisphere (Hatfield, PA)
|
| |
|
| |
| Hybridization protocol |
Samples were hybridised using the Array 900microRNA direct kit from Genisphere (Hatfield, PA) as described by the manufacturer on a A-hyb machine (Miltenyi Biotec).
|
| Scan protocol |
Slides were scanned on a Perkin-Elmer ArrayExpress scanner
|
| Description |
Image analysis was carried out with BlueFuse software (BlueGnome, Cambridge, UK)
|
| Data processing |
Raw image data were global median-normalized within arrays and normalized between arrays using the LIMMA package. The normalized log ratios (average of four replicates per probe) were used for subsequent analysis in Genespring 7.2 (Agilent Technologies, CA, US).
|
| |
|
| Submission date |
May 05, 2010 |
| Last update date |
May 11, 2010 |
| Contact name |
charles lawrie |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Oxford
|
| Department |
NDCLS
|
| Street address |
John Radcliffe Hospital
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10385 |
| Series (1) |
| GSE21697 |
MicroRNA expression in Sézary syndrome: Identification, function and diagnostic potential. |
|
