|
| Status |
Public on Sep 22, 2011 |
| Title |
CD1 MEFs_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Mouse embryonic fibroblasts
|
| Treatment protocol |
2i conversion was performed using MEK inhibitor (2 μM) and GSK inhibitor (12 μM) from StemGent in LIF supplemented ES media.
|
| Growth protocol |
Low passage primary MEFs were grown in DMEM (Wisent) supplemented with 10% FBS (Wisent), 10 μg/mL penicilin and streptomysin (Sigma), and 2 mM L-glutamine (Sigma). J1 ES cells and iPS cell clones were grown on arrested feeder cells under standard conditions in the presence of LIF. EOS3F-24, -28, and -29 iPS cells were mantained under 1 μg/ml puromycin selection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Whole transcript sense target labeling assay
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
n/a
|
| Data processing |
The data were analyzed with RMA as background correction, summarization & normalization method.
|
| |
|
| Submission date |
Apr 29, 2010 |
| Last update date |
Sep 22, 2011 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE21595 |
Comparisons between fully and partially reprogrammed iPS cells induced by pMX-Klf4, pMX-Oct4 and pMX-Sox2 retroviruses |
|
