|
| Status |
Public on Apr 28, 2010 |
| Title |
zebrafish 12hpf Ovo1 morphant-2 |
| Sample type |
RNA |
| |
|
| Source name |
zebrafish embryos 12 hpf
|
| Organism |
Danio rerio |
| Characteristics |
tissue type: whole embryo
strain: AB
genotype/variation: Ovo1 morphant, sox10(7.2):gfp
age: 12 hpf (6 somites)
|
| Growth protocol |
Embryos were maintained in a 28.5oC incubator until 12 hpf (6 somites)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix GeneChip Expression Analysis Technical Manual, 2002)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC with rotation on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using a GeneChip ® Scanner 3000.
|
| Description |
gene expression of wild type embryos injected with Ovo1 morpholino-2
|
| Data processing |
The results were quantified and analyzed using Expression Console ver. 1.1 software (Affimetrix, Inc.) using the PLIER algorithm default values.
|
| |
|
| Submission date |
Apr 27, 2010 |
| Last update date |
Jun 24, 2010 |
| Contact name |
Sarah Piloto |
| Organization name |
University of California, Irvine
|
| Department |
Developmental and Cell Biology
|
| Lab |
Thomas Schilling
|
| Street address |
4462 Natural Sciences II, Dept. Dev. and Cell Biology
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE21539 |
Comparison of expression data between control and Ovo1 morphant zebrafish embryos |
|
