|
| Status |
Public on May 25, 2022 |
| Title |
MEFs, WT_02 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Embyonic fibroblast
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: WT
cell type: MEFs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Kit (QIAGEN) and on column DNase digestion was performed according to manufacturer protocol. Quality control was performed using Agilent Bioanalyzer.
|
| Label |
Standard Illumina protocol using Cy3.
|
| Label protocol |
Biotinylated cRNA were prepared with TargetAmpTM- Nano labelling Kit for illumina Expression Beadchip
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
By using illumina Bead Array Reader version 3.7.9.27572
|
| Description |
replicate 2
|
| Data processing |
The raw data was extracted from illumina's Genome studio software and the same used to check the quality of data. The data were normalized using quantile normalization method.
|
| |
|
| Submission date |
Jun 09, 2021 |
| Last update date |
May 25, 2022 |
| Contact name |
Soumen Basak |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Immunology
|
| Department |
Immunology
|
| Lab |
Systems Immunology Lab
|
| Street address |
Aruna Asaf Ali Road
|
| City |
Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platform ID |
GPL15097 |
| Series (1) |
| GSE176446 |
Genome-wide analysis of Wild Type and Relb-/- MEFs |
|
