|
| Status |
Public on May 02, 2022 |
| Title |
Control 1-week old rep5 |
| Sample type |
SRA |
| |
|
| Source name |
Heads
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: W1118
tissue: fly heads
age: 1 week
|
| Treatment protocol |
Aged the Gba1b-/- flies and their W1118 controls to 1 week and 3 weeks on standard SYA food.
|
| Growth protocol |
SYA food
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Flies were flash frozen in liquid nitrogen, and decapitated on dry ice. RNA was harvested using Trizol reagent. Samples were polyA enriched using the Kapa mRNA HyperPrep kit. Illumina NextSeq v2.5, 150 cycles was used with 120ng of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
G5_S21_R1/R2_combined.fastq.gz
|
| Data processing |
Reads mapping to the Drosophila genome (downloaded from FlyBase.org, version dmel_r6.31_FB2019_06).
Reads sorting by gene name.
Gene counts was performed with featureCounts (v.2.0.0) (https://doi.org/10.1093/bioinformatics/btt656) [default parameters] using the transcriptome (downloaded from FlyBase.org, version dmel_r6.31_FB2019_06/gtf).
Differential gene experssion using DESeq2 (https://doi.org/10.1186/s13059-014-0550-8).
Genome_build: dmel_r6.31_FB2019_06
Supplementary_files_format_and_content: raw counts
|
| |
|
| Submission date |
May 24, 2021 |
| Last update date |
May 02, 2022 |
| Contact name |
Kerri Kinghorn |
| E-mail(s) |
[email protected]
|
| Organization name |
University College London (UCL)
|
| Department |
Genetics, Evolution and Environment
|
| Lab |
Institute of Healthy Ageing
|
| Street address |
Gower Street
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN19317443 |
| SRA |
SRX10971683 |
