Tissue RNA was isolated using Qiagen RNeasy kits (Qiagen, Inc., Valencia, CA). Biotinylated-cRNA made from pooled RNA preparations (RNA from two rats per preparation) was used to probe Affymetrix Rat Expression 230A GeneChips (Affymetrix) as per the Invitrogen protocol (Invitrogen, Inc., Carlsbad, CA), three arrays per group.
Label
biotin
Label protocol
Biotin-labeled cRNA probes were in vitro transcribed using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Life Sciences).
Hybridization protocol
Standard Affymetrix protocol.
Scan protocol
Standard Affymetrix protocol.
Description
n/a
Data processing
Data analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method
