|
| Status |
Public on May 20, 2021 |
| Title |
Control siRNA Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Control siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
disease: Colorectal carcinoma
sirna sequence: GAUCAUACGUGCGAUCAGAdTdT
duration: 72h
|
| Treatment protocol |
Synthetic short interfering RNA (siRNA) oligonucleotides (Sigma) were delivered into cells with Lipofectamine RNAi Max (Invitrogen). 40 nM of siRNA oligonucleotides were transfected using the reverse transfection protocol according to the manufacturer’s instructions.
|
| Growth protocol |
Cells were cultured at 37° C with 5% CO2 in Dulbecco's modified Eagle's medium (DMEM) high glucose (Hyclone) supplemented with 5% fetal bovine serum (FBS, Hyclone) and penicillin-streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent
|
| Label |
Biotin
|
| Label protocol |
Standard cRNA biotin labeling protocol for illumina microarray
|
| |
|
| Hybridization protocol |
Standard hybridization protocol for illumina microarray
|
| Scan protocol |
Standard scanning protocol for illumina microarray
|
| Description |
Replicate #1
|
| Data processing |
All analyses were performed using GenomeStudio software (Illumina, Inc.)
|
| |
|
| Submission date |
May 19, 2021 |
| Last update date |
May 20, 2021 |
| Contact name |
Han Liao |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Texas Health Science Center at Houston
|
| Street address |
6431 Fannin St.
|
| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE174729 |
Analysis of gene expression change in cancer cells induced by nucleolar stress |
|
