|
| Status |
Public on Apr 01, 2022 |
| Title |
KO 2 |
| Sample type |
SRA |
| |
|
| Source name |
Liver endothelium
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
genotype: c-Maf KO
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
ECs were isolated by fluorescence-activated cell sorting and subjected to RNA extraction using RNeasy Mini Kit from Qiagen.
RNA-seq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
MAF_KO_MCR_149_S59_L005_R1_001
|
| Data processing |
FastQC (v0.11.5) to check FASTQ quality.
Skewer (v0.2.2) for adapter trimming
Minion from Kraken tool (reaper-13-100) for adapter diagnosis
STAR (v2.7.6a) for alignment and gene count quantification using --quantMode GeneCounts
Genome_build: GRCm38
Supplementary_files_format_and_content: Gene count matrix
|
| |
|
| Submission date |
Apr 19, 2021 |
| Last update date |
Apr 01, 2022 |
| Contact name |
Jesus Maria Gomez Salinero |
| E-mail(s) |
[email protected]
|
| Organization name |
Weill Cornell Medicine
|
| Street address |
1300 York Avenue
|
| City |
NEW YORK |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE172360 |
Temporal and functional determinants of intra-hepatic vascular homeostatic specialization [mouse liver] |
| GSE172362 |
Temporal and functional determinants of intra-hepatic vascular homeostatic specialization |
|
| Relations |
| BioSample |
SAMN18797634 |
| SRA |
SRX10637049 |
