|
| Status |
Public on Feb 17, 2011 |
| Title |
NY222 |
| Sample type |
RNA |
| |
|
| Source name |
Human hepatocellular carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
vascular invasion: Yes
bclc_staging: B
age: 60
Sex: M
code: resection
class: CTNNB1
afp: 2.6
tp53 mutation: 0
ctnnb1 mutation: 232G>GT | D32D/Y
immunohistochemistry: pRPS6 : 1
immunohistochemistry: pIGFR1 : 0
immunohistochemistry: pAkt : 0
immunohistochemistry: CTNNB1 : 0
used for prognostic prediction: yes
used for microrna profiling: Yes
microRNA-based subclass: A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using fresh frozen HCC tissues, total RNA was extracted using TRIzol (Invitrogen).
|
| Label |
Biotin
|
| Label protocol |
Described in Nature 2005 Jun 9;435(7043):834-8. PMID: 15944708
|
| |
|
| Hybridization protocol |
Described in Nature 2005 Jun 9;435(7043):834-8. PMID: 15944708
|
| Scan protocol |
Described in Nature 2005 Jun 9;435(7043):834-8. PMID: 15944708
|
| Description |
NY222
|
| Data processing |
After subtracting background signal intensities, data were normalized by median scaling, then a floor value of 20 was applied.
|
| |
|
| Submission date |
Mar 02, 2010 |
| Last update date |
May 04, 2011 |
| Contact name |
Yujin Hoshida |
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL10122 |
| Series (2) |
| GSE20594 |
microRNA profiling of human hepatocellular carcinoma |
| GSE20596 |
MicroRNA-Based Classification of Hepatocellular Carcinoma and Oncogenic Role of miR-517a |
|
