|
| Status |
Public on Jun 12, 2022 |
| Title |
Sham Il1b |
| Sample type |
SRA |
| |
|
| Source name |
Mouse brain
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 3 months
gender: male
treatment: Sham Il1b
tissue: brain
|
| Treatment protocol |
Injection of artificial CSF (sham), or injection of Visudyne (5 uL) followed by 5 steps of transcranial photoconversion (meningeal lymphatic vessel ablation). Each combined with i.p. injection of either 1μg IL-1β or saline vehicle.
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10x Genomics Chromium single cell preparation
NGS Illumina Next Seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
RNA was isolated from the brains of 5 mice.
|
| Data processing |
Illumina bcl2fastq software used for basecalling
UMI count matrices generated using the Cellranger count pipeline with force_counts set to 2000. The output from the cellranger count pipeline for each sample has been uploaded as a folder containing count, barcode, and gene matrices.
Genome_build: mm10
Supplementary_files_format_and_content: Compressed tar archives containing the raw UMI count matrices for each sample, as well as matrices with gene symbols and barcodes.
|
| |
|
| Submission date |
Mar 11, 2021 |
| Last update date |
Jun 12, 2022 |
| Contact name |
Jonathan Kipnis |
| Organization name |
Washington University in Saint Louis
|
| Department |
Pathology and Immunology
|
| Lab |
Kipnis Lab
|
| Street address |
4515 McKinley Ave
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE168755 |
Age-associated suppression of exploratory activity during sickness is linked to meningeal lymphatic dysfunction and microglia activation [ablation] |
| GSE168756 |
Age-associated suppression of exploratory activity during sickness is linked to meningeal lymphatic dysfunction and microglia activation |
|
| Relations |
| BioSample |
SAMN18269836 |
| SRA |
SRX10323355 |
