|
| Status |
Public on Jul 07, 2010 |
| Title |
Leukemia, Immunophenotype mixed lineage leukemia (MLL) |
| Sample type |
RNA |
| |
|
| Source name |
hematopoietic stem/precursor cells transduced with AF4-MLL and MLL-AF4 that developed leukemia
|
| Organism |
Mus musculus |
| Characteristics |
disease state: leukemia
cell type: leukemic cells (isolated from bone marrow of diseased mouse)
immunophenotype: mixed lineage leukemia (MLL)
|
| Treatment protocol |
Cells were not sorted rather than anylzed by FACS fro their immunophenotype; 3 different immunophenotypes were revealed
|
| Growth protocol |
Bone marrow cells were isolated after several month from moribund mice that developed leukemia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Rneasy mini kit (QIAGEN)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix standard protocol (2 µg RNA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip MG-430_2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned in a GeneChip Scanner 3000 (G7 update)
|
| Description |
Gene expression data from leukemic cells with an MLL immmunophenotype
|
| Data processing |
The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 24, 2010 |
| Last update date |
Jul 07, 2010 |
| Contact name |
Rolf Marschalek |
| E-mail(s) |
[email protected]
|
| Phone |
049-69-798-29647
|
| Fax |
049-69-798-29647
|
| Organization name |
Goethe University
|
| Department |
Inst Pharm Biology
|
| Street address |
Max-von-Laue-Str. 9
|
| City |
Frankfurt/Main |
| ZIP/Postal code |
60438 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE20519 |
Gene expression analysis of leukemic samples in C57BL6 mice |
|
