|
| Status |
Public on Mar 28, 2011 |
| Title |
bcatGOF mouse kidney E12.5, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
mutant mouse embryonic kidney day 12.5
|
| Organism |
Mus musculus |
| Characteristics |
genotype: ß-catenin gain-of-function mutant (ß-catGOF-UB)
tissue: kidney (uteric bud)
developmental stage: embryo
age: 12.5 days
|
| Treatment protocol |
tissue was disected, imaged by digital microscopy and randomly sorted into one of three sample vials
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were stored in RNAlater RNA stabilization reagent (Qiagen, Canada) and then isolated using the RNeasy Micro kit (Qiagen, Canada).
|
| Label |
biotin
|
| Label protocol |
one cycle amplification as per Affymetrix protocol from 1ug of total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse MOE430v2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Hoxb7-Cre:EGFP mice ( Zhao, et al. (2004) Dev Biol 276:403-415) were crossed with mice containing loxP sites flanking exon 3 of the ß-catenin allele (ß-catdelta3/delta3) (Harada,et al. (2002) Cancer Res 62:1971-1977) to generate ß-catenin gain-of-function mutant mice specifc to the uteric bud, termed ß-catGOF-UB .Eighteen ß-catGOF-UB mutant kidneys and 9 WT kidneys were micro-dissected at E12.5. Mutant kidneys were divided into three random pools (n=3) consisting of 6 kidneys each.
Expression data for mouse ß-catGOF-UB mutant kidneys embryonic day E12.5
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Feb 14, 2010 |
| Last update date |
Mar 28, 2011 |
| Contact name |
Brian Joseph Cox |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Toronto
|
| Department |
Physiology
|
| Lab |
Cox System Biology
|
| Street address |
1 King's College Circle, Rm 3360
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S1A8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE20325 |
Stabilization of ß-catenin during murine Kidney Development |
|
