|
| Status |
Public on Feb 11, 2010 |
| Title |
lung microvessel 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
endothelial cell
|
| Organism |
Homo sapiens |
| Characteristics |
source: non-brain derived
cell line: HLMEC
|
| Treatment protocol |
Cells were not treated.
|
| Growth protocol |
Endothelial cells were cultured in gelatin-coated 24 well culture plates in an endothelial cell growth medium containing 10%FBS, 1% Pen/Strep, 1% l-glutamine, 1X heparin and 60 mg/L endothelial cell grwoth supplement in M199.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen Rneasy kit
|
| Label |
Cy5
|
| Label protocol |
Ambion MessageAmpII Protocol - 1 round Amplification
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human reference
|
| Organism |
Homo sapiens |
| Characteristics |
source: Stratagene Universal Human reference
|
| Treatment protocol |
Cells were not treated.
|
| Growth protocol |
Endothelial cells were cultured in gelatin-coated 24 well culture plates in an endothelial cell growth medium containing 10%FBS, 1% Pen/Strep, 1% l-glutamine, 1X heparin and 60 mg/L endothelial cell grwoth supplement in M199.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen Rneasy kit
|
| Label |
Cy3
|
| Label protocol |
Ambion MessageAmpII Protocol - 1 round Amplification
|
| |
|
| |
| Hybridization protocol |
see www.genome.duke.edu for standard hybridization protocol
|
| Scan protocol |
scan according to Axon GenePix 4000B recommendations
|
| Description |
n/a
|
| Data processing |
Data were normalized in GeneSpring to the Stratagene Universal Human reference control (Cy3 channel)
|
| |
|
| Submission date |
Feb 09, 2010 |
| Last update date |
Feb 10, 2010 |
| Contact name |
Heather A Himburg |
| E-mail(s) |
[email protected]
|
| Phone |
919-681-2042
|
| Fax |
919-668-4777
|
| Organization name |
Duke University Medical Center
|
| Department |
Medicine/Cellular Therapy
|
| Lab |
John Chute
|
| Street address |
PO BOX 91013
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL5770 |
| Series (1) |
| GSE20243 |
Identification of soluble factors overexpressed in brain endothelial cell (EC) lines compared to non-brain EC lines |
|
