|
Status |
Public on Jul 31, 2011 |
Title |
VIHCC P_M235 |
Sample type |
RNA |
|
|
Source name |
Human hepatocellular carcinoma
|
Organism |
Homo sapiens |
Characteristics |
tissue: hepatocellular carcinoma vascular invasion: No
|
Extracted molecule |
total RNA |
Extraction protocol |
Using formalin-fixed, paraffin-embedded HCC tissues, total RNA was extracted using TRIzol (Invitrogen).
|
Label |
Cy5
|
Label protocol |
Refer to the Illumina beadarray Assay System Manual
|
|
|
Hybridization protocol |
Refer to the Illumina beadarray Assay System Manual
|
Scan protocol |
Refer to the Illumina beadarray Assay System Manual
|
Description |
none
|
Data processing |
For each probe, replicate mesurements were averaged after removing outliers. After subtracting background signal intensities and applying a floor value of 0.01 was assigned to negative values, data were normalized using the cubic spline algorithm implemented in GenePattern IlluminaNormalizer module.
|
|
|
Submission date |
Jan 22, 2010 |
Last update date |
Jul 31, 2011 |
Contact name |
Yujin Hoshida |
Organization name |
University of Texas Southwestern Medical Center
|
Street address |
5323 Harry Hines Blvd
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75390 |
Country |
USA |
|
|
Platform ID |
GPL8432 |
Series (2) |
GSE20017 |
Gene Signature to Identify Vascular Invasion in Hepatocellular Carcinoma |
GSE20238 |
Gene Signature to Identify Vascular Invasion in Human Hepatocellular Carcinoma |
|
Relations |
Reanalysis of |
GSM499178 |