|
| Status |
Public on Feb 17, 2011 |
| Title |
P_B215 |
| Sample type |
RNA |
| |
|
| Source name |
Human hepatocellular carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: hepatocellular carcinoma
vascular invasion: N/A
used for qpcr of microrna in chr19q13.41: Yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using formalin-fixed, paraffin-embedded HCC tissues, total RNA was extracted using TRIzol (Invitrogen).
|
| Label |
Cy5
|
| Label protocol |
Refer to the Illumina beadarray Assay System Manual
|
| |
|
| Hybridization protocol |
Refer to the Illumina beadarray Assay System Manual
|
| Scan protocol |
Refer to the Illumina beadarray Assay System Manual
|
| Description |
none
|
| Data processing |
For each probe, replicate mesurements were averaged after removing outliers. After subtracting background signal intensities, data were then normalized using the cubic spline algorithm.
|
| |
|
| Submission date |
Jan 20, 2010 |
| Last update date |
Feb 17, 2011 |
| Contact name |
Yujin Hoshida |
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL8432 |
| Series (3) |
| GSE19977 |
Gene-expression profiles of formalin-fixed, paraffin-embedded human hepatocellular carcinoma |
| GSE20140 |
Combining Clinical, Pathology, and Gene Expression Data to Predict Recurrence of Hepatocellular Carcinoma |
| GSE20596 |
MicroRNA-Based Classification of Hepatocellular Carcinoma and Oncogenic Role of miR-517a |
|
