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Sample GSM49886 Query DataSets for GSM49886
Status Public on Dec 20, 2005
Title intermediolateral column motoneurons (IML) 18, sham-operated control
Sample type RNA
 
Source name adult spinal cord, sham-operated control
Organism Mus musculus
Characteristics cell type: intermediolateral column motoneuron (IML)
Extracted molecule total RNA
 
Description Three cell types, intermediolateral column motoneurons, medial motoneurons, and lateral motoneurons were isolated from a single adult spinal cord using laser capture microscopy. Four hundred captures were collected for each cell type. For a given cell type, RNA was extracted from the 400 captures using an Arcturus picopure kit. RNA was split in half and two targets were produced using a double amplification protocol. Each target was hybridized to Affymetrix chips and signals were normalized with R-pack. Inverse logs are provided. Five animals were used in these experiments, and all three cell types were collected from each animal. Thus, for each cell type, there are five biological replicates, and for each biological replicate there are two technical replicates. In all thirty chips were analyzed. Techinical replicates are indicated as Set 1 and Set 2. Animal numbers are indicated by Pair1 through Pair 5.
This sample is also known as sham mice. Set 2 - Pair 3 - IML
 
Submission date Apr 28, 2005
Last update date Sep 22, 2009
Contact name Deborah Baro
E-mail(s) [email protected]
Organization name Georgia State University
Street address P.O. Box 4010
City Atlanta
State/province GA
ZIP/Postal code 30303
Country USA
 
Platform ID GPL81
Series (2)
GSE2595 Intermediolateral column, medial, and lateral motoneurons
GSE18179 Gene profiling of intermediolateral column, medial, and lateral motoneuron after spinal cord transection

Data table header descriptions
ID_REF Probeset ID
VALUE RMA Express calculated the Signal values

Data table
ID_REF VALUE
100001_at 37.208518
100002_at 571.310319
100003_at 26.445326
100004_at 179.449352
100005_at 181.222787
100006_at 82.083953
100007_at 257.272583
100009_r_at 929.801744
100010_at 58.474438
100011_at 58.770408
100012_at 668.30732
100013_at 89.947732
100014_at 133.766768
100015_at 154.767229
100016_at 44.454135
100017_at 49.247775
100018_at 191.791152
100019_at 533.010265
100020_at 225.726925
100021_at 32.577493

Total number of rows: 12488

Table truncated, full table size 250 Kbytes.




Supplementary file Size Download File type/resource
GSM49886.CEL.gz 2.8 Mb (ftp)(http) CEL

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