|
| Status |
Public on Aug 31, 2021 |
| Title |
F2 Embry.63 T CM mi 367 Mu |
| Sample type |
RNA |
| |
|
| Source name |
Musculus longissimus dorsi tissue from 63 dpc pig
|
| Organism |
Sus scrofa |
| Characteristics |
breed: F2 based German Landrace (DL) x Pietrain (Pi)
tissue: Musculus longissimus dorsi
age: 63 days post conception (dpc)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen miReasy Mini kit TRI Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol for small-RNA. Then small RNA was cleaned up using the Rneasy MinElute Clean up kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from miRNA according to FlashTag Biotin RNA Labeling Kit for Affymetrix GeneChip miRNA arrays (Genisphere, Hatfield, PA, USA) according to the manufacturer’s recommendations Affymetrix GeneChip miRNA Array protocols (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip miRNA arrays. GeneChips were washed and processed to detect biotin-containing transcripts by Streptavidin-PE (Phycoerythrin) conjugate.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Description |
microRNA expression data from skeletal muscle of 63 dpc of F2 population based breed Pi and DL.
Muscle samples of foetuses (F2) derived from the F1 sows were collected and evaluated at gestational day 63. Muscle (longissimus dorsi) samples were immediately frozen in liquid nitrogen and stored at -80°C until RNA extraction.
|
| Data processing |
Data were analysed with Expression Console and quantify by med polish
|
| |
|
| Submission date |
Dec 07, 2020 |
| Last update date |
Aug 31, 2021 |
| Contact name |
Siriluck Ponsuksili |
| E-mail(s) |
[email protected]
|
| Organization name |
FBN
|
| Street address |
Wilhelm-stahl-allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14969 |
| Series (1) |
| GSE162755 |
Gene expression data of micro RNA from skeletal muscle sample of F2 population based German Landrace (DL) and Pietrain (Pi) pig breeds |
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