|
| Status |
Public on Jan 29, 2011 |
| Title |
FR-si221-3 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 fulvestrant-resistant cell line transfected by antisense hsa-miR-221
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: si221
cell line: MCF7-FR
|
| Treatment protocol |
For transfection assays, cells were plated in antibiotic-free media and transfected with Lipofectamine 2000 (Invitrogen, Carlasbad, CA) according to the manufacturer's instructions. Antagomirs were transfected at 100nM concentrations.
|
| Growth protocol |
MCF7-FR cells were growing in phenol red-free medium with 10% charcoal-stripped fetal bovine serum (FBS) and 10E-7 mol/L fulvestrant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
FR-si221-3
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using R default settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jan 07, 2010 |
| Last update date |
Jan 29, 2011 |
| Contact name |
Meng Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Indiana University
|
| Department |
Medical Sciences
|
| Street address |
1001 E 3rd Street
|
| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19777 |
Antisense miRNA-221/222 (si221/222) and control inhibitor (GFP) treated fulvestrant-resistant breast cancer cells |
|
