|
| Status |
Public on Jan 11, 2021 |
| Title |
RH001_NEU_DM |
| Sample type |
RNA |
| |
|
| Source name |
Diabetic Neurones
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Laser captured cells from human temporal cortex
disease state: Diabetic material
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Immuno directed laser capture microdissection was used to isolate total RNA from neurones, astrocytes and endothelial cells.
RNA was isolated using the Arcturus PicoPure RNA isolation kit according to manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylation of antisense RNA carried out according to Affymetrix IVT protocol
|
| |
|
| Hybridization protocol |
12µg of cRNA molecules were heat fragmented and applied to the Human U133 plus 2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
|
| Scan protocol |
The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the AGCC software package to prepare the .CEL files
|
| Description |
D3
Gene expression data from human temporal cortex neurones
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console v4.1.1 (Affymetrix) using Robust Multi-Array Average (RMA) as normalization method.
|
| |
|
| Submission date |
Nov 12, 2020 |
| Last update date |
Jan 11, 2021 |
| Contact name |
Paul Roy Heath |
| E-mail(s) |
[email protected]
|
| Phone |
+44 114 2222254
|
| Organization name |
University of Sheffield
|
| Department |
Academic Neurology Unit
|
| Lab |
Heath
|
| Street address |
SITraN, 385a Glossop Road
|
| City |
Sheffield |
| State/province |
S. Yorks |
| ZIP/Postal code |
S10 2HQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE161355 |
Type 2 Diabetes Mellitus is Associated with Transcriptome Alterations in Cortical Neurones and Associated Neurovascular Unit Cells in the Ageing Brain |
|
