|
| Status |
Public on Apr 22, 2010 |
| Title |
FPC88 12454_Neurosphere Cultures vs. reference GE2-v5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
SRCID:SRC122569,PRBID:PRB21779,FILE:Agilent_251485025150_S01_GE2-v5_95_Feb07_1_3.txt
|
| Organism |
Homo sapiens |
| Characteristics |
source: UCSF
sample id: FPC88
cell type: 12454_Neurosphere Cultures
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen's AllPrep DNA/RNA extraction kit (Catalog #80204) was used to extract DNA and RNA according to manufacturer's protocol
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Linear Amplification
|
| |
|
| Channel 2 |
| Source name |
Universal Human Reference from Stratagene (#740000)
|
| Organism |
Homo sapiens |
| Characteristics |
type: reference
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
None
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Linear Amplification
|
| |
|
| |
| Hybridization protocol |
According to manufacturer's protocol (Agilent)
|
| Scan protocol |
According to manufacturer's protocol (Agilent)
|
| Description |
SRCID:SRC122569,PRBID:PRB21779,FILE:Agilent_251485025150_S01_GE2-v5_95_Feb07_1_3.txt
|
| Data processing |
Data were normalized using Agilent feature extraction software v9.5, with the Stratagene Universal Human Reference in the reference channel, according to manufacturer's protocol. Ratios are Cy5/Cy3, representing test/ref.
|
| |
|
| Submission date |
Dec 23, 2009 |
| Last update date |
Apr 22, 2010 |
| Contact name |
Anthony Steve Guerrero |
| Organization name |
Genentech
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE19612 |
A Hierarchy of Self-Renewing Tumor-Initiating Cell Types in Glioblastoma |
|
