|
| Status |
Public on Apr 21, 2011 |
| Title |
Sample 39 - MLL/AF10 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: AML samples
cells: Peripheral Blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the standard Affymetrix IVT express protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000 7G
|
| Description |
Gene expression data from acute myeloid leukemia with MLL/AF10 translocations at diagnosis
|
| Data processing |
The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org]
|
| |
|
| Submission date |
Dec 18, 2009 |
| Last update date |
Apr 21, 2011 |
| Contact name |
Andrea Zangrando |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Padova
|
| Department |
Woman and Child's Health
|
| Lab |
Hemato-Oncology
|
| Street address |
Via Giustiniani, 3
|
| City |
Padova |
| ZIP/Postal code |
35138 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19577 |
MLL partner genes confer distinct biological and clinical signatures of pediatric AML, an AIEOP study |
|
