|
| Status |
Public on Jul 01, 2010 |
| Title |
WT_OD=0.5_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
WT strain, OD(600)=0.5
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotpye: leu2, trp1, gal2, ura3-52, leu2-3, prb1-1122, pep4-3, prc467, CCT6::CBP::KanMX4
|
| Treatment protocol |
The cells were then pelletted by centrifugation for 1 min at 600 g, flash-frozen at -80 °C in liquid N2 and stored at -80 °C until use
|
| Growth protocol |
Overnight cultures of the wild-type and each of the MA strains were diluted appropriately and then grown until an O.D. of 0.5 was reached.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the MasterPure Yeast RNA purification kit (Epicentre Biotechnologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized to the GeneChip Yeast Genome 2.0 array for 16 hrs at 45 °C with shaking.
|
| Scan protocol |
The chips were scanned using a GeneChip 3000 scanner.
|
| Description |
Gene expression data from WT strain grown to OD(600)=0.5
|
| Data processing |
analysis was carried out using Partek Genome Suite software (Partek Inc., St. Louis, Missouri 63141, http://www.partek.com), using RMA.
|
| |
|
| Submission date |
Dec 16, 2009 |
| Last update date |
Dec 16, 2009 |
| Contact name |
Amnon Horovitz |
| E-mail(s) |
[email protected]
|
| Phone |
972-8-9343399
|
| Fax |
972-8-9344188
|
| Organization name |
Weizmann Institue of Science
|
| Department |
Structural Biology
|
| Lab |
Horovitz
|
| Street address |
P.O.Box 26
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE19511 |
Equivalent mutations in the eight subunits of the chaperonin CCT produce dramatically different cell phenotypes. |
|
