|
| Status |
Public on Oct 28, 2020 |
| Title |
PAO1_MIC/10_Exp5_t=24 (DNA-seq) |
| Sample type |
SRA |
| |
|
| Source name |
bacteria
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
strain: PAO1 (Wild Type)
treatment: MIC/10
time: 24hr
|
| Treatment protocol |
solution was removed by centrifugation and bacteria were stored at -20°C prior to DNA extraction
|
| Growth protocol |
Hydroponic experiment in Growth Minimal medium (Rathnayake, I. V. N.; Megharaj, M.; Krishnamurti, G. S. R.; Bolan, N. S.; Naidu, R., Heavy metal toxicity to bacteria – Are the existing growth media accurate enough to determine heavy metal toxicity? Chemosphere. 2013, 90, (3), 1195-1200.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was harvested using QIAamp® DNA Mini Kit QIAGEN
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
45-Sample
|
| Data processing |
Library strategy: WGS
Quality control assessment using FastQC
Data processing (trimming using AdapterRemoval, Alignment using BWA, variant calling using snippy)
Genome_build: GCF_000006765.1_ASM676v1
Supplementary_files_format_and_content: Variant call format (VCF)
|
| |
|
| Submission date |
Oct 27, 2020 |
| Last update date |
Oct 28, 2020 |
| Contact name |
James Breen |
| E-mail(s) |
[email protected]
|
| Organization name |
Australian National University
|
| Department |
John Curtin School of Medical Research
|
| Lab |
Black Ochre Data Labs (Adelaide)
|
| Street address |
108 North Terrace (Ground Floor)
|
| City |
Adelaide |
| State/province |
SA |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL21297 |
| Series (2) |
| GSE160188 |
Do Copper enhance antibiotic resistance (AR) in resistant bacteria and/or induce AR in sensitive bacteria? [DNA-seq] |
| GSE160190 |
Do Copper enhance antibiotic resistance (AR) in resistant bacteria and/or induce AR in sensitive bacteria? |
|
| Relations |
| BioSample |
SAMN16562344 |
| SRA |
SRX9370189 |
