|
| Status |
Public on Jan 24, 2021 |
| Title |
pilot_W171_HEK293T_R1 |
| Sample type |
SRA |
| |
|
| Source name |
Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell_line: HEK293T
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction by Norgen total RNA Kit or RNA-XP beads.
C19-SparSeq-Barcoded multiplexed PCR reactions were pooled and cleaned with SPRIselect beads.
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
SparSeq multiplexed PCR library
Pilot_ReadCount_Table.csv
|
| Data processing |
Single-end reads mapped to amplicon sequences using Bowtie and allowing 3 mismatch for mapped read count / amplicon quantification.
Genome_build: Custom genome consists of amplicons targeted in GRCh38 and Sars-CoV-2 (Wuhan-Hu-1).
Supplementary_files_format_and_content: Sample name/ read count mapped to each targeted amplicon.
|
| |
|
| Submission date |
Oct 25, 2020 |
| Last update date |
Jan 24, 2021 |
| Contact name |
Jeff Wrana |
| E-mail(s) |
[email protected]
|
| Organization name |
Lunenfeld Tanenbaum Research Institute
|
| Street address |
600 Unv Ave
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1X5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL21697 |
| Series (2) |
| GSE160033 |
A Multiplexed, Next-Generation Sequencing Platform for High-Throughput Detection of SARS-CoV-2 [pilot cohort] |
| GSE160036 |
A Multiplexed, Next-Generation Sequencing Platform for High-Throughput Detection of SARS-CoV-2 |
|
| Relations |
| BioSample |
SAMN16541014 |
| SRA |
SRX9354640 |
