The sample is a non-responder to imatinib treatment and belongs in the training set from Leipzig. The sample originated from blood or bone marrow from patient with chronic myelogenous leukemia (CML), before imatinib treatment. Red blood cells were removed by red cell lysis buffer. Either guanidine-thiocyanate / phenol-cholorform extraction followed by RNeasy (Leipzig), or cesium chloride-gradient purification (Mannheim) were used to extract total RNA from the patient’s white blood cells. Labeling and hybridization is done by standard Affymetrix protocols. Scanning is done by HP GeneArray Scanner and image analysis is carried out by Affymetrix Microarray Suite Software, version 5.0. Robust Multipchip Analysis (RMA) was used for signal quantification, on Bioconductor (http://www.bioconductor.org). Keywords = chronic myelogenous leukemia Keywords = imatinib Keywords = Gleevec Keywords = cytogenetic responses Keywords = Affymetrix
