|
| Status |
Public on Dec 11, 2009 |
| Title |
48 H KLF2 EXPRESSION,ISOLATE 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
UMBILICAL VEIN ENDOTHELIAL CELL
|
| Organism |
Homo sapiens |
| Characteristics |
time: 48 H
cell type: umbilical vein endothelial cell
isolate: 1
protocol: KLF2-transduced
|
| Treatment protocol |
Cells were transduced by lentiviral vectors expressing no protein (mock) or Krueppel-like factor 2, and total RNA was isolated at the indicated times 24, 48 and 72 h after transduction
|
| Growth protocol |
Human umbilical vein endothelial cells were grown in fibronectin coated tissue culture, using standard medium M199+20% FBS + ECGS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Stratagene RNAeasy kits and QC by Agilent Bioanalyzer, RIN scores > 8
|
| Label |
Cy3
|
| Label protocol |
RNA was amplified and aminoallyl-labelled using Ambion MessageAmp kit, then Labelled with Cy3 and Cy5 monoreactive dyes
|
| |
|
| Channel 2 |
| Source name |
UMBILICAL VEIN ENDOTHELIAL CELL
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: umbilical vein endothelial cell
isolate: 1
protocol: mock-transduced
time: 48 H
|
| Treatment protocol |
Cells were transduced by lentiviral vectors expressing no protein (mock) or Krueppel-like factor 2, and total RNA was isolated at the indicated times 24, 48 and 72 h after transduction
|
| Growth protocol |
Human umbilical vein endothelial cells were grown in fibronectin coated tissue culture, using standard medium M199+20% FBS + ECGS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Stratagene RNAeasy kits and QC by Agilent Bioanalyzer, RIN scores > 8
|
| Label |
Cy5
|
| Label protocol |
RNA was amplified and aminoallyl-labelled using Ambion MessageAmp kit, then Labelled with Cy3 and Cy5 monoreactive dyes
|
| |
|
| |
| Hybridization protocol |
hyb chamber, overnight, 37 C, 40% formaldehyde
|
| Scan protocol |
Agilent-II scanner
|
| Description |
KLF2 TRANSDUCED CELLS COMPARED TO MOCK TRANSDUCED CELLS 48 H AFTER LENTIVIRAL TRANSDUCTION
|
| Data processing |
Feature extraction by ScanArray v6, values excluded if <2x noise, then loes normalized ratios KLF2/mock samples (Limma package Bioconductor) were processed by interarray Quantile normalization (Bioconductor)
|
| |
|
| Submission date |
Dec 11, 2009 |
| Last update date |
Dec 11, 2009 |
| Contact name |
Anton J Horrevoets |
| URL |
http://www.vumc.nl/afdelingen/moleculairecelbiologie
|
| Organization name |
VU University Medical Center
|
| Department |
Molecular Cell Biology
|
| Lab |
B244
|
| Street address |
van der Boechorststraat 7
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 BT |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL4868 |
| Series (1) |
| GSE19412 |
KLF2-induced actin shear fibers control both alignment to flow and JNK signaling in vascular endothelium |
|
