|
| Status |
Public on Jul 02, 2010 |
| Title |
S. aureus-AFN-1252-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
S. aureus (RN4220) treated with AFN-1252
|
| Organism |
Staphylococcus aureus |
| Characteristics |
strain: RN4220 (derivative of NCTC8325)
|
| Treatment protocol |
S. aureus cells were treated with AFN-1252 (50 ng/mL) or and equal volume in DMSO from 15 minutes
|
| Growth protocol |
S. aureus strain RN4220 was grown to an OD at 600nm = 0.4 in 1% tryptone broth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Ambion RNAqueous purification kit according to the manufacturer's instructions and the purified RNA sample was treated with Turbo Dnase (Ambion).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on a GeneChip S. aureus Genome array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data for S. aureus treated with AFN-1252
|
| Data processing |
The data were analyzed with GeneChip Operating Software version 1.4 using the default MAS 5.0 algorithm settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 09, 2009 |
| Last update date |
Dec 09, 2009 |
| Contact name |
Matthew Wilmer Frank |
| E-mail(s) |
[email protected]
|
| Phone |
901-595-5614
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Infectious Diseases
|
| Lab |
Dr. Charles Rock
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL1339 |
| Series (1) |
| GSE19400 |
S. aureus gene expression following AFN-1252 treatment |
|
