|
| Status |
Public on Feb 26, 2010 |
| Title |
case 3 mRNA |
| Sample type |
RNA |
| |
|
| Source name |
GCT patient case
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary intracranial pediatric germ cell tumor (GCT)
diagnosis: germinoma
age: 14.2 years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from CNS GCT patient
|
| Data processing |
RMA through R based affymetrix module
|
| |
|
| Submission date |
Dec 07, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yu Hsuan Wu |
| E-mail(s) |
[email protected]
|
| Organization name |
National Yang-Ming University
|
| Street address |
No.155, Sec.2, Linong Street
|
| City |
Taipei |
| ZIP/Postal code |
112 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE19348 |
expression data from pediatric primary intracranial germ cell tumor |
| GSE19350 |
Array-based bioinformatic analysis on pediatric primary central nervous system germ cell tumors |
|
| Relations |
| Reanalyzed by |
GSE119087 |
