|
| Status |
Public on Dec 03, 2009 |
| Title |
Aorta_APOE-deficient with atherosclerosis_1 |
| Sample type |
RNA |
| |
|
| Source name |
Aortas isolated from atherosclerotic APOE-deficient mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype: APOE-deficient
tissue: aorta
treatment: none
disease: atherosclerosis
age: 32-34 weeks
|
| Treatment protocol |
The study was performed with aortas isolated from three groups of mice (32-34 weeks of age), i.e. untreated APOE-deficient mice (APOE), APOE-deficient mice treated with captopril (20 mg/kg in drinking water) for 7 months (Capto), and nontransgenic control mice (Cont).
|
| Growth protocol |
The study was initiated with 4-6 week-old APOE-deficient mice on a C57BL/6J background, and non-transgenic C57BL/6J controls. Mice had free access to food and water, and were fed a standard rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). For atherosclerosis treatment, one subgroup of APOE-deficient mice received captopril in drinking water (20 mg/kg) for 7 months. At an age of 32-34 weeks, aortas were isolated from three study groups (APOE-deficient, captopril-treated APOE-deficient, and nontransgenic control mice), dissected free of fat, and immediately frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total aortic RNA was isolated with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
|
| |
|
| Hybridization protocol |
For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybridization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Description |
Gene expression data from atherosclerotic aortas isolated from APOE-deficient mice.
APOE-1
|
| Data processing |
The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
|
| |
|
| Submission date |
Dec 02, 2009 |
| Last update date |
Dec 02, 2009 |
| Contact name |
Ursula Quitterer |
| E-mail(s) |
[email protected]
|
| Phone |
+41-446329801
|
| Organization name |
ETH Zurich
|
| Department |
Molecular Pharmacology
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
CH-8057 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE19286 |
Microarray gene expression profiling of aorta genes of APOE-deficient mice receiving the ACE inhibitor captopril |
| GSE42813 |
Microarray gene expression profiling of aortic genes of APOE-deficient mice receiving atherosclerosis treatment with the antioxidant vitamin E |
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