|
| Status |
Public on Dec 05, 2009 |
| Title |
Wenzel_1-2_NPA-treated-2wk-shoots_Rep1_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
Col-0, NPA
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
developmental stage: 1.04
tissue: shoot
stock code: N1092
treatment: NPA
|
| Growth protocol |
Seeds of Arabidopsis thaliana ecotype Col-0 were sterilized with chlorine gas and plated onto sterile ATS media (Lincoln et al., 1990) supplemented with either N-(1-Naphthyl) phthalamic acid (NPA; Sigma, St. Louis, MO) dissolved in DMSO to a final concentration of 10 mM, or an equivalent volume of DMSO for control material. Seeds were stratified for at least 2 days at 4C and then grown at ca 20C with 16h illumination for 2 weeks (shoot material). Shoot tissues were immediately immersed in liquid nitrogen after excision, stored at -80C, and total RNA purified according to Chang et al. (1993). Lincoln et al. 1990. Plant Cell 2: 1071-1080 Chang et al. 1993. Biology Reporter 11: 113-116
|
| Extracted molecule |
total RNA |
| Extraction protocol |
see Chang et al. 1993. Biology Reporter 11: 113-116
|
| Label |
biotin
|
| Label protocol |
Labelled using protocol described in manual "Affymetrix GeneChip Expression Analysis Technical Manual", One-Cycle target labelling
|
| |
|
| Hybridization protocol |
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
|
| Scan protocol |
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.
|
| Description |
4C for at least 2 days 3 hr chlorine gas Growth Room Agar 70% 20oC Own medium: see Lincoln et al. 1990. Plant Cell 2: 1071-1080. Modification: add N-(1-Naphthyl) phthalamic acid (NPA; Sigma, St. Louis, MO) dissolved in DMSO to a final concentration of 10 uM. Shoot tissue from ca 2wk old plants with ca 6 rosette leaves.
|
| Data processing |
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
|
| |
|
| Submission date |
Dec 02, 2009 |
| Last update date |
Dec 04, 2009 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE19262 |
NPA-induced vascular overgrowth in Arabidopsis |
|
