|
| Status |
Public on Feb 01, 2010 |
| Title |
Ramos siCTR_4 |
| Sample type |
RNA |
| |
|
| Source name |
Burkitt lymphoma cell line transduced with lentivirus expressing a control siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Burkitt lymphoma cell line
|
| Treatment protocol |
The cells were infected with lentiviral particles expressing a commercial control siRNA (SHC002, Sigma)
|
| Growth protocol |
Cells were lysated in Trizol Reagent (Invitrogen) and frozen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's indications.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for at least 16 hr at 45C on GeneChip Human Genome Array U95Av2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
Gene expression data from Burkitt lymphoma cell line transduced with lentivirus expressing a control siRNA
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 30, 2009 |
| Last update date |
Nov 30, 2009 |
| Contact name |
Katia Basso |
| E-mail(s) |
[email protected]
|
| Phone |
212-8515269
|
| Fax |
212-8515256
|
| Organization name |
Columbia University
|
| Department |
Institute for Cancer Genetics
|
| Street address |
1150 St. Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE2350 |
Normal and transformed human mature B cells |
|
