|
| Status |
Public on Mar 01, 2010 |
| Title |
human_LIMA_artery_patient5_bare_metal_cardiovascular_stent |
| Sample type |
RNA |
| |
|
| Source name |
Human LIMA artery from patient 5, treatment: bare metal cardiovascular stent
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
individual: patient 5
treatment: bare metal cardiovascular stent
|
| Treatment protocol |
Thereafter, a bare metal stent (Express2, 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted (1 min, 10 bar) into one of the three LIMA pieces and a paclitaxel-eluting stent (TAXUS Express2 2.5 x 16 mm stent, Boston Scientific Corporation, MA, USA) was implanted into another. The third part was left as unstented control. Forty-eight hours post stent implantation, the stents were removed and the LIMA samples snap frozen in liquid nitrogen.
|
| Growth protocol |
As part of elective Coronary Artery Bypass Graft Surgery procedures performed at Lund University Hospital, Sweden, tissues containing parts of the LIMA from four coronary artery bypass graft (CABG) patients (68-73 year old males with stable angina and 3-vessel coronary atheroslerosis, two had hypertension, none were diabetic) were collected and stored in DMEM low glucose at 4C. Each LIMA (n=4) was dissected free of surrounding tissues, cut into three equal parts and incubated under tissue culture conditions in serum-free DMEM. All LIMA vessel segments were approximately 2 mm in diameter. The vessel segments were incubated for 24 h to induce quiescence.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were homogenized in TRIzol and RNA purified as described in Amisten et al 2008 (PMID 17920662).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Hu133Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
stable angina and 3-vessel coronary atheroslerosis
|
| Data processing |
The data were analyzed with Expression Console ver.1 using Affymetrix default analysis settings followed by MAS5 as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Nov 23, 2009 |
| Last update date |
Nov 23, 2009 |
| Contact name |
Ann-Sofie Albrekt |
| E-mail(s) |
[email protected]
|
| Phone |
+46462229818
|
| Organization name |
Lund University
|
| Street address |
Solvegatan 19
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19136 |
Gene expression response to implanted drug (paclitaxel)-eluting or bare metal stents in denuded human LIMA arteries |
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