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| Status |
Public on Feb 11, 2022 |
| Title |
Motoneuron_P3A3 |
| Sample type |
SRA |
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| Source name |
Motoneuron
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| Organism |
Drosophila melanogaster |
| Characteristics |
population: Ok371>RFP+ DAPI- DRAQ5+
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Collections of 19-20h old Drosophila embryos were collected and dissociated. Cells were stained with Dapi and DRAQ5 to exclude debris. Single cells were sorted into 96-well-plates in Smart-seq2 lysis buffer. Full-length cDNA from individual cells were produced following the SmartSeq2 protocol.
Smart-Seq2 (modified from Picelli et al., Nature Protocols 2014) Tagmentation using Homemade Tn5 (Hennig et al., G3 2018)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
Adapter trimming, perl
Pseudoalignment, kallisto 0.43.0
Count tables, R 3.5.1/scater 1.10.1
Genome_build: Drosophila melanogaster, BDGP6 ensemble
Supplementary_files_format_and_content: Kallisto output (tsv)
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| Submission date |
Aug 03, 2020 |
| Last update date |
Feb 11, 2022 |
| Contact name |
Jessica Velten |
| Organization name |
University of Heidelberg
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| Department |
Centre for Organismal Studies
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| Lab |
Lohmann lab
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| Street address |
Im Neuenheimer Feld 230
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platform ID |
GPL19132 |
| Series (2) |
| GSE155578 |
single-cell RNA-seq of embryonic Drosophila motoneurons |
| GSE155587 |
single-cell RNA-seq of embryonic Drosophila |
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| Relations |
| BioSample |
SAMN15703612 |
| SRA |
SRX8871380 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM4706575_Motoneuron_P3A3.tsv.gz |
273.3 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
| Raw data are available in SRA |
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